Research Interests - Laboratory of Clinical Virology

Published in: Cellular Physiology and Biochemistry.

The role of RhoA, RhoB and RhoC GTPases in cell morphology, proliferation and migration in human cytomegalovirus (HCMV) infected glioblastoma cells.

Melpomeni Tseliou1, Ahmed Al-Qahtani2, Saud Alarifi3, Saad Η. Alkahtani3, Christos Stournaras4*, George Sourvinos1*

* These authors share equal senior authorship:
1Laboratory of Virology, Medical School, University of Crete, Heraklion, Crete, Greece.
2Department of Biological & Medical Research, Research Center, King Faisal Hospital & Research Center, Riyadh, Saudi Arabia.
3Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
4Department of Biochemistry, Medical School, University of Crete, Heraklion, Crete, Greece.


Video legend:
U373MG and U373MG-IE1 cell motility after depletion of RhoA, RhoB or RhoC in uninfected or HCMV infected cells.
Both the parental U373MG and the derivatives U373MG-IE1 cells were transduced with the indicated shRNA TRIPZ lentivirus vectors and subsequently infected with HCMV (MOI=3 pfu/cell ) or not.
After 48 hours of infection (mock or HCMV), images were acquired by time-lapse microscopy every 15 min for 5 h.
The replay speed is 200 min/s.